This what I did to add a separator to ASP.Net menu. I used Web.sitemap to bind the data to menu control. This how Web.sitemap looks like. <sitemap xmlns="http://schemas.microsoft.com/AspNet/SiteMap-File-1.0"> <sitemapnode> <sitemapnode url="~/Pages/DatingHome.aspx" title="Home" description="" separatorImageUrl="../../Images/but_blueSlice.jpg"> <sitemapnode url="~/Pages/Inbox.aspx" title="Inbox" description="" separatorImageUrl="../../Images/but_blueSlice.jpg"> </sitemapnode> </sitemapnode> This separatorImageUrl will tell you the image path of the separator. This event handler will do the rest of it. protected void Menu2_MenuItemDataBound(object sender, System.Web.UI.WebControls.MenuEventArgs e) { SiteMapNode smNode = (SiteMapNode)e.Item.DataItem; if (smNode["seperatorImageUrl"] != null) e.Item.SeparatorImageUrl = smNode[...